Technology Overview

The LCSB team works in close collaboration with platform users and research groups to support instrument use, quality control, data acquisition, analysis, and interpretation. Specific expertise in image analysis enables us to assist users with the development of custom-made software tools.

The facility’s focus is to grant researchers access to state-of-the-art analytical imaging technologies. As such, the BIP comprises advanced microscopy systems for high resolution studies of fixed and live samples as well as for low-phototoxicity imaging of whole organs and organisms. Image analysis is supported by strong in-house capacities and the university’s high-performance computing facility. Platform users are trained to become proficient on individual systems.

Services Offered

Training and access to instruments:

Super-resolution Leica SP8 STED 3X, Confocal Zeiss LSM 710, Spinning Disk Zeiss Cell Observer, High Content Perkin Elmer Opera, Automated epi-fluorescence Nikon Eclipse Ti-E and Olympus IX83 with multiple light sources
Selective Plane Illumination Microscope constructed in-house with four laser lines (405, 488, 561, and 633 nm), three objectives (20x water, 25x water with ultra long working distance, 40x water), and high-precision 4D stage
Becton Dickinson FACSAriaIII cell sorter, Becton Dickinson LSRFortessa cell analyzer
Training on advanced microscopy and flow cytometry systems
Support for fixed sample, tissue, and live-cell imaging

Opportunity

Access to the platform under collaborative service agreements is possible on demand.

LCSB Bioimaging Platform